Application

GenElute^™Water RNA/DNA Purification Kit - 0.45 μm has been used:in total RNA extraction from formalin-fixed paraffin-embedded (FFPE) tissue sectionsto extract total RNA from paraffin-embedded non-neoplastic lymphatic tissue and neoplastic lymph node tissuesto extract total RNA

Bioanalyzer Quantitative, real-time RT-PCR for large RNA and small RNA including miRNA RT-PCR for large RNA and small RNA including miRNA Northern blotting RNase protection Primer extension Expression array assays Next Generation Sequencing microRNA Cloning miRNA array RNA-Seq Nanostring Fluidigm Droplet/Digital PCR

Features and Benefits

High quality and integrity of the isolated RNA - The purified total RNA is of the highest quality and integrity, and can be used in any sensitive downstream applications. Isolate a diversity of RNA species - All RNA species can be isolated, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). High yields - GenElute’;s FFPE RNA Purification Kit allows for the purification of high yields of total RNA. No phenol:chloroform extractions - Total RNA is isolated from FFPE tissue samples without the use of harmful chemicals such as phenol or chloroform. Rapid procedure - Isolate total RNA from FFPE tissue sections using a rapid spin column format in as little as 1 hour

General description

Sigma’;s GenElute^™ FFPE RNA Purification Kit provides a rapid method for the isolation and purification of total RNA (including microRNA) from formalin-fixed paraffin-embedded (FFPE) tissue samples. Using formalin to fix tissues leads to crosslinking of the RNA and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the RNA over time. Sigma’;s GenElute^™ FFPE RNA Purification Kit provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of RNA. The kit is able to purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as the degree of fragmentation of the RNA will increase over time. The RNA is preferentially purified from other cellular components without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including qRT-PCR, reverse transcription PCR, primer extension, expression array assays, and microarray analyses. Extract total RNA (including microRNA) from FFPE samples No phenol extraction step Includes DNase for optional on-column DNA removal Isolated RNA is of the highest quality and integrity

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Other Notes

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Unit Definition

Preps